SETMAR protein-GST fusion
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Datasheet

SETMAR protein-GST fusion

009-001-T30S 009-001-T30
20 µg 50 µg
Human
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$309.00 /Per Item
$629.00 /Per Item
20 µg $309.00
50 µg $629.00
Availability: Ships in approximately 30 days Availability: Ships in approximately 30 days
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Description

Background

SETMAR or SET domain and mariner transposase fusion gene is a nonhomologous end-joining repair protein that regulates genomic integration of exogenous DNA by opening chromatin and facilitating joining of DNA ends (1). SETMAR has histone methyltransferase activity and methylates 'Lys-4' and 'Lys-36' of histone H3. SETMAR also has DNA nicking activity and may play a role in DNA repair (2). Human Pso4 forms a stable complex with SETMAR that regulates Metnase function in DNA repair. SETMAR has sequence-specific DNA-binding activity and recognizes the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element. SETMAR Protein is ideal for investigators involved in Signaling Proteins, Acetyl/Methyltransferase Proteins, Apoptosis/Autophagy, Cancer, Cell Cycle, and Neurobiology research.

Application Note

SETMAR Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. SETMAR Protein is suitable for use in Western Blot. Expect a band approximately ~66-69kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Purity/Specificity

Recombinant full-length human SETMAR was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.

SETMAR recombinant protein-GST fusion protein - 009-001-T30
METNASE, Histone-lysine N-methyltransferase SETMAR, HsMar1, Histone-lysine N-methyltransferase, Mariner transposase Hsmar1
SETMAR
Human
Sf9 cells
Recombinant Protein
Liquid (sterile filtered)
0.2 µg/µL
See application note.
Dry Ice
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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