SEPHAROSE™ PROTEIN G
SEPHAROSE™ Protein G - Bottle
Protein G Sepharose - Western Blot Box Images
SEPHAROSE™ Protein G - Antibody Figure
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Datasheet

SEPHAROSE™ PROTEIN G

PG50-00-0002
2 mL
Streptococcus sp.
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$229.00 /Per Item
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$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

Protein G is prepared by covalently coupling recombinant Protein G to 6% crosslinked sepharose beads. The coupling technique is optimized to give a high binding capacity for IgG, resulting in IgG binding greater than 10 mg of human IgG per cc of slurry. Protein G is used for immunoprecipitation and purification of monoclonal and polyclonal antibodies. This product shows stability within pH range of 2 to 10, is high flow rate capable, and has low falling off of rProtein G.

Application Note

Protein G resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.

Purity/Specificity

Sepharose™ Protein G binds to all IgG subclasses from human, monkey, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.

SEPHAROSE™ PROTEIN G - PG50-00-0002
ProG, Streptococcus G protein, Sepharose Protein G
Streptococcus sp.
Sepharose™
greater than 20mg/ml human IgG bound per CC Sepharose
Agarose Conjugate
Suspension of agarose beads
drained sepharose gel (~1 cc settled sepharose beads per 2 ml suspension)
Wet Ice
Store SEPHAROSE PROTEIN G at 4° C prior to opening. DO NOT FREEZE. Supplied in 20% Ethanol/Water
Expiration date is one (1) year from date of opening.
Zuo, Y et al. Contributions of the RhoA guanine nucleotide exchange factor Net1 to polyoma middle T antigen-mediated mammary gland tumorigenesis and metastasis. Breast Cancer Research : Bcr (2018)
Applications
IP, Co-IP

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.