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Datasheet Protocol

Sepharose™ Protein A/G

6 References
PAG50-00-0002
2 mL
Suspension of agarose beads
ChIP, IP
Staphylococcus aureus, Streptococcus sp.
$99.00 /Per Item
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Background

Sepharose™ Protein A/G can be used for immunoprecipitation and purification of monoclonal antibodies. Sepharose™ Protein A/G is a suspension of sepharose beads containing binding domains from both protein A (from Staphylococcus aureus) and protein G (from Streptococcus sp.). When evenly dispersed approximately 2.0 ml of suspension is equal to 0.5 cc of settled beads of Sepharose™ 4 Fast Flow (highly cross linked, 4% agarose derivative). The bead size is approximately 45-165 µm when swollen. Each cc of drained beads will bind approximately 20-30 mg Human IgG. The exclusion limit (Mr) of the beads is 3 x 10^7. The maximum linear flow rate is approximately 1300 cm/hr. This product shows long term stability within pH range of 3 to 9 and short term stability within pH range of 2 to 10. Up to 100 reactions per 2ml.

Product Details

Sepharose™ Protein A/G - PAG50-00-0002
Sepharose Protein A/G, Protein AG, agarose, ProA-ProG, Staphylococcus A protein, Sepharose Protein A, Streptococcus G protein, Sepharose Protein G, immunoprecipitation, IP western blot
Staphylococcus aureus, Streptococcus sp.
Sepharose™
Recombinant Protein

Target Details

The product can be used for binding IgG from human, rabbit, goat, mouse and rat. It has strong binding of human, rabbit, cow, sheep, and goat polyclonal antibodies, mouse IgG2a, IgG2b, IgG3, and rat IgG2a. Protein A/G also has moderate affinity for mouse and rat IgG1 and IgG2c. It binds with weak affinity to rat IgG2b. Sepharose™ Protein A/G can be used for immunoprecipitation and purification of monoclonal antibodies.
P38507 - UniProtKB

Application Details

ChIP, IP  - View References
Protein A/G resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.

Formulation

0.5cc drained Sepharose per 2ml slurry
20% (v/v) Ethanol

Shipping & Handling

Wet Ice
Store SEPHAROSE PROTEIN A/G at 4° C prior to opening. DO NOT FREEZE.
Expiration date is one (1) year from date of receipt.
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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.