rHuman Flt-3 Ligand Protein
SDS-PAGE of Human Flt-3 Ligand Recombinant Protein
SDS-PAGE of Human Flt-3 Ligand Recombinant Protein
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Datasheet

rHuman Flt-3 Ligand Protein

009-001-U86-0002 009-001-U86-0010 009-001-U86-0100 009-001-U86-1000
2 µg 10 µg 100 µg 1 mg
SDS-PAGE, Cellular Assay
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$109.00 /Per Item
$260.00 /Per Item
$679.00 /Per Item
$5,615.00 /Per Item
2 µg $109.00
10 µg $260.00
100 µg $679.00
1 mg $5,615.00
Availability: Ships in approximately 30 days Availability: Ships in approximately 30 days Availability: Ships in approximately 30 days Availability: Ships in approximately 30 days
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Description

Background

FMS-related Tyrosine Kinase 3 Ligand (FLT-3 Ligand) is a growth factor important for the proliferation of hematopoietic cells. FLT-3 Ligand binds to, and transmits signals through, the receptor tyrosine kinase known as FMS-like Tyrosine Kinase-3 (FLT-3). FLT-3 Ligand promotes long-term expansion and differentiation of human pro-B cells in the presence of IL-7 or in combination of IL-7 and IL-3. Human FLT-3 Ligand can stimulate the proliferation of cells expressing murine FLT-3 receptors. Recombinant human FLT-3 Ligand is a non-glycosylated protein, containing 155 amino acids, with a molecular weight of 17.7 kDa.

Application Note

Flt-3 Ligand Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti- Flt-3 Ligand in immunological assays. Buffer Formulation: 10 mM sodium phosphate and 50 mM sodium chloride, pH 7.5.

Purity/Specificity

Flt-3 Ligand purity was determined to be greater than 98% as determined by analysis by HpLC, UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Human Flt-3 Ligand Recombinant Protein - 009-001-U86-0002
FLt3 L, Fms-related tyrosine kinase 3 ligand
FLT3LG
SDS-PAGE
Cellular Assay
Human
Recombinant Protein
Lyophilized
0.2 mg/ml
See application note.
None
None
2µl (2-20µl)
Restore with deionized water (or equivalent)
Ambient
Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
Expiration date is six (6) months from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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