Rel B protein-HIS Epitope
no-image-default.jpg
Datasheet is currently unavailable. Try again or CONTACT US
Datasheet

Rel B protein-HIS Epitope

009-001-T18S 009-001-T18
20 µg 50 µg
Human
Have a Question? Ask a Specialist
$298.00 /Per Item
$579.00 /Per Item
20 µg $298.00
50 µg $579.00
Availability: Ships in approximately 30 days Availability: Ships in approximately 30 days
Shipping info:

$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

Rel B is part of the NFkB complex and forms heterodimeric complexes with p50 (NFKB1) and p52 (NFKB2) (1). The homodimeric complexes of Rel B alone do not show DNA-binding activity. IHC analysis has shown that Rel B expression correlated with dendritic cell activation. NFkB-inducing kinase NIK is required for osteoclastogenesis in response to pathologic stimuli and overexpression of Rel B rescues differentiation of mouse NIK -/- osteoclast precursors. Rel B is required for RANKL-induced osteoclastogenesis in vitro and for TNF -induced bone resorption in vivo. This indicates that the alternative NFkB pathway, via Rel B, plays an essential and unique role in RANKL signaling toward osteoclast development (2). Rel B Protein is ideal for investigators involved in Signaling Proteins, Transcription Proteins, Apoptosis/Autophagy, Cancer, Inflammation, Neurobiology, and NFkB Pathway research.

Application Note

Rel B Protein is stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. Rel B Protein is suitable for use in Western Blot. Expect a band approximately ~68kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Purity/Specificity

Recombinant full-length human Rel B was expressed by baculovirus in Sf9 insect cells using an N-Terminal his epitope. The purity was determined to be >85% by densitometry.

Rel B recombinant protein-HIS Epitope - 009-001-T18
IREL, I-REL, Transcription factor RelB
RELB
Human
Sf9 cells
Protein
Recombinant Protein
Liquid (sterile filtered)
0.2 µg/µL
See application note.
Dry Ice
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Expiration date is one (1) year from date of opening.

Invalid lot number

If you need help finding your CofA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Related products to: Rel B protein-HIS Epitope