Rat IgG1 isotype control Fluorescein
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Datasheet

Rat IgG1 isotype control Fluorescein

Monoclonal RG1 IgG1

012-002-040
50 µg
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$116.00 /Per Item
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Description

Background

RAT IgG1 isotype control is used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In Rat there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 2c and μ - IgM, light chain kappa and lambda.

Application Note

RAT IgG1 isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.

Purity/Specificity

RAT IgG1 isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rat IgG, and anti-Rat serum.  Isotyping assay resulted non-reactive with antisera to Rat IgG2a, IgG2b, IgG3, IgM, and IgA. Fluorescein Conjugated Rat IgG1 confirmed by Dot Blot.

RAT IgG1 isotype control Fluorescein conjugated - 012-002-040
Rat Isotype Control, Rat IgG1 FITC isotype, RAT IgG1 subclass isotype control
Fluorescein (FITC)
Monoclonal
RG1
IgG1
2-8 moles FITC per mole of IgG1
Protein
Native Protein
Liquid (sterile filtered)
1.0 by UV absorbance at 280 nm
0.01% (w/v) Sodium Azide
None
Wet Ice
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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