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Datasheet

PFKFB3 protein-GST fusion

009-001-S88S 009-001-S88
20 µg 50 µg
Liquid (sterile filtered) Liquid (sterile filtered)
Human
Sf9 cells
$325.00 /Per Item
$665.00 /Per Item
Availability: Ships in approximately 30 days Availability: Ships in approximately 30 days
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

PFKFB3 or 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 is a potent activator of a key regulatory enzyme in the glycolysis pathway called phosphofructokinase. Phosphofructokinase regulates the steady-state concentration of fructose-2, 6-bisphosphate, a potent activator of key regulatory enzyme of the glycolysis pathway. PFKFB3 is bifunctional, although the phosphatase activity is low due to a mutation in an active site which can be upregulated after phosphorylation by protein kinases (1). PFKFB3 may be responsible for sustaining the high glycolytic flux of rapidly proliferating leukemia cells and may represent a molecular target for chemotherapeutic intervention (2). PFKFB3 Protein is ideal for investigators involved in Signaling Proteins, Fructose Kinase Proteins, Cancer, Cell Cycle, and Metabolic Disorder research.

Product Details

PFKFB3 recombinant protein-GST fusion protein - 009-001-S88
IPFK2, PFK2, ATP-dependent 6-phosphofructokinase subunit beta, ATP-dependent 6-phosphofructokinase 2, ATP-PFK 2, Phosphofructokinase 2, Phosphohexokinase 2
Human
Sf9 cells
Recombinant Protein

Target Details

PFKFB3
Recombinant full-length human PFKFB3 was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.

Application Details

PFKFB3 Protein is stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. PFKFB3 Protein is suitable for use in Western Blot. Expect a band approximately ~83kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Formulation

0.1 µg/µL
See application note.

Shipping & Handling

Dry Ice
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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