p27KIP1 protein-GST fusion
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Datasheet

p27KIP1 protein-GST fusion

009-001-172S 009-001-172
20 µg 50 µg
Human
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$298.00 /Per Item
$579.00 /Per Item
20 µg $298.00
50 µg $579.00
Availability: Ships in approximately 30 days Availability: Ships in approximately 30 days
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Description

Background

KIP1 (cyclin-dependent kinase inhibitor 1B) is a kinesin-related motor protein required for mitotic spindle assembly and chromosome segregation (1). Many tumorigenic processes modulate cell-cycle progression by regulating the levels of the cyclin-dependent kinase inhibitor KIP1. KIP1 binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (2). The phosphorylation- and ubiquitination-dependent proteolysis of KIP1 is implicated in control of the G1-S transition in the cell cycle. KIP1 is critical for retinoblastoma protein (Rb)-induced cellular proliferative senescence. p27KIP1 Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.

Application Note

p27KIP1 Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. p27KIP1 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~52kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Purity/Specificity

Recombinant full-length human p27KIP1 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.

p27KIP1 recombinant protein-GST fusion protein - 009-001-172
CDKN1B, CDKN4, MEN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1
CDKN1B
Human
E. coli
Protein
Recombinant Protein
Liquid (sterile filtered)
0.2 µg/µL
See application note.
Dry Ice
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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