Micrococcal Nuclease S7
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Datasheet

Micrococcal Nuclease S7

MB-116-0015
15 ku
Staphylococcus aureus
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$168.00 /Per Item
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Description

Background

Micrococcal nuclease catalyzes cleavage of both DNA and RNA to yield 3'-nucleotides. It exhibits exo- and endo-5'-phosphodiesterase activities. The enzyme catalyzes preferential endohydrolysis of the RNA and DNA at sites rich in adenylate or uridylate and deoxyadenylate or thymidylate. The enzyme has a molecular weight of 16,807 daltons and is calcium dependent. The pH optimum is 9.2 but varies depending upon the concentration of ionized calcium present.

Application Note

One Unit causes a change in optical density of 1.0 at 260 nm at 37°C, pH 8.0, using DNA as the substrate.

Purity/Specificity

Purified by chromatography and assayed by SDS-PAGE.

Micrococcal Nuclease (S7) - MB-116-0015
Staphylococcus aureus
Enzyme
≥6,000 units per mg protein
Lyophilized
1.0 mL
Restore with deionized water (or equivalent)
Wet Ice
Store vial at 4° C or at -20° C or colder prior to opening product.
Expiration date is six (6) months from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.