MDM2 (1-118) protein-HIS Epitope
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Datasheet

MDM2 (1-118) protein-HIS Epitope

009-001-S45S 009-001-S45
20 µg 50 µg
Human
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$165.00 /Per Item
$256.00 /Per Item
20 µg $165.00
50 µg $256.00
Availability: Ships in approximately 30 days Availability: Ships in approximately 30 days
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$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

MDM2 is a nuclear phosphoprotein that binds and inhibits transactivation by p53, as part of an autoregulatory negative feedback loop (1). Overexpression of the MDM2 gene product can lead to excessive inactivation of p53 thereby diminishing its tumor suppressor function. The inactivation of p53 is mediated by the E3 ubiquitin ligase activity of MDM2 which targets p53 for proteasomal degradation. MDM2 also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5 (2). Amplification of MDM2 is frequently observed in human sarcomas and this is consistent with the hypothesis that MDM2 binds to p53 which then leads to escape from p53-regulated growth control. MDM2 Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, AKT/PKB Pathway, Apoptosis/Autophagy, Cancer, Cell Cycle, and Cellular Stress research.

Application Note

MDM2 Protein is stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. MDM2 Protein is suitable for use in Western Blot. Expect a band approximately ~17.5kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Purity/Specificity

Recombinant human MDM2 (1-118) was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >85% by densitometry.

MDM2(1-118) recombinant protein-HIS Epitope - 009-001-S45
HDMX, hdm2, MGC71221, E3 ubiquitin-protein ligase Mdm2
MDM2
Human
E. coli
Protein
Recombinant Protein
Liquid (sterile filtered)
0.2 µg/µL
See application note.
Dry Ice
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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