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KDM5B protein-GST fusion

010-001-S36S 010-001-S36
20 µg 50 µg
Liquid (sterile filtered) Liquid (sterile filtered)
Sf9 cells
$325.00 /Per Item
$665.00 /Per Item
Availability: Ships in approximately 30 days Availability: Ships in approximately 30 days
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$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.


KDM5B is a lysine (K)-specific demethylase 5B which is highly expressed in testis and shows the lowest expression in skeletal muscle (1). KDM5B is a histone H3 lysine 4 (H3K4) demethylase up-regulated in prostate cancer and associated with androgen receptor where it regulated its transcriptional activity in reporter gene assays (2). Overexpression of KDM5B in HeLa cells results in loss of tri-, di-, and monomethyl H3K4, but does not alter the methylation status of H3K9 or H3K36. A KDM5B containing highly conserved putative DNA/chromatin binding motif is specifically up-regulated in breast cancer. KDM5B Protein is ideal for investigators involved in Signaling Proteins, Deacetylase/Demethylase Proteins, Cancer, Cell Cycle, and Inflammation research.

Product Details

KDM5B recombinant protein-GST fusion protein - 010-001-S36
Jarid1b,PUT1,Plu1,PLU-1,Rb-Bp2, AW556288, RBBP2H1A, mKIAA4034, Lysine-specific demethylase 5B, EC 1.14.11.-, Histone demethylase JARID1B, Jumonji/ARID domain-containing protein 1B, PLU-1
Sf9 cells
Recombinant Protein

Target Details

Recombinant mouse KDM5B (1-753) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.

Application Details

KDM5B Protein is stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. KDM5B Protein is suitable for use in Western Blot. Expect a band approximately ~112kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.


0.1 µg/µL
See application note.

Shipping & Handling

Dry Ice
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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