Human IgM (myeloma) Fc5u
SDS PAGE Results of Human IgM Fc5µ
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Datasheet

Human IgM (myeloma) Fc5u

009-0131
1 mg
SDS-PAGE
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Description

Background

Human IgM (myeloma) Fc5µ fragment consists of only the µ (mu) chain of the Fc fragment. Immunoglobulin M is the largest antibody isotype and the first to be secrected against an initial exposure to antigen. IgM is predominantly produced in the spleen. IgM is formed from covalently linking 5 immunoglobulins together. Due to this large size, IgM is typically isolated to the serum.

Application Note

Human IgM (myeloma) Fc5µ fragment has been tested in SDS-Page and can be used in Western Blotting and ELISA experiments as a control reagent.

Purity/Specificity

Human IgM (myeloma) Fc5µ fragment has been prepared from Human IgM myeloma protein by digestion with trypsin followed by column chromatography.  Purity was assessed by SDS-PAGE and HPLC to be greater than 95%.  A single precipitin arc was observed against anti-human IgM Fc5µ and anti-human serum when assayed by immuno-electrophoresis at a concentration of 20 mg/ml.  No reaction was observed against anti-Trypsin, anti-human IgG F(ab')2, anti-human IgG F(c), anti-human Kappa or anti-human Lambda.

HUMAN IgM (myeloma) Fc5µ fragment - 009-0131
Human immunoglobulin M fragment; Human IgM Fc5µ
SDS-PAGE
Human
IgM
Protein
Native Protein
Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
0.05% (w/v) Sodium Azide
Wet Ice
Store vial at 4° C prior to opening.   Human IgM (myeloma) Fc5µ fragment is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.
Expiration date is one (1) year from date of opening.

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