Datasheet

HSP27 protein-GST fusion

Name: HSP27 protein-GST fusion
Brand: Rockland Immunochemicals
Price: 325.0 USD
Availability: OutOfStock

Item No. 009-001-234

Size: 50 µg

Origin: Human

Expressed in: E. coli

$665.00 /Per Item

20 µg $325.00

50 µg $665.00

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Background

HSP27 is a member of heat shock proteins (HSPs) which are synthesized in cells in response to heat shock and other metabolic stresses and provide a transient state of thermotolerance. HSP27 plays a major role in the increased thermal resistance acquired by cells after exposure to HSP inducers (1). The level of HSP27 phosphorylation is significantly elevated after exposure of cells to heat shock, sodium arsenite, IL-1 and TNF-a. MAPKAPK2 and MAPKAPK3 are both activated by these conditions and can phosphorylate HSP27 on serine residues (2). HSP27 Protein is ideal for investigators involved in Signaling Proteins, Cell Stress & Chaperone Proteins, Cancer, Cellular Stress, and p38 Pathway research.

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Specifications for HSP27 protein-GST fusion

Product Details

Description: HSP27 recombinant protein-GST fusion protein - 009-001-234

Synonyms: CMT2F, HSPB1, HSP28, Hsp25, HS.76067, DKFZp586P1322, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein

Species of Origin: Human

Expressed in: E. coli

Type: Recombinant Protein

Target Details

Gene Name: HSPB1

Purity/Specificity: Recombinant full-length human HSP27 expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >80% by densitometry.

Database Links:

NCBI - NM_001540

Application Details

Application Note: HSP27 Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. HSP27 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~52kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 0.2 µg/µL

Buffer: See application note.

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiration: Expiration date is one (1) year from date of receipt.

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.