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Datasheet

Deoxyribonuclease I

MB-101-0100 MB-101-0500
100 Units 500 Units
Liquid (sterile filtered) Liquid (sterile filtered)
Bovine
$94.00 /Per Item
$210.00 /Per Item
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Background

Deoxyribonuclease I is an endonuclease which splits phosphodiester linkages yielding polynucleotides with a free 3’-OH and 5’-phosphate. Activated by bivalent metal ions and inhibited by chelators such as EDTA. Chromatographically purified to be free of ribonuclease and protease activity.

Product Details

Deoxyribonuclease I (Bovine Pancreas) RNAse and Protease Free Molecular Biology Grade - MB-101-0100
Deoxyribonuclease I reagent, Bovine Pancreas Deoxyribonuclease I
Bovine
Enzyme
2,400 Kunitz/mL

Target Details

Enzyme Concentration: 2290 units/ml or ~2.2 units/ul (100 units/vial) which approximately equals 1 mg/ml. % Ribonuclease: None detected % Protease: None detected

Application Details

One unit will cause an increase in the absorbance at 260 nm of 0.001 per minute per ml at 25° C, pH 5.0 when acting on highly polymerized DNA according to the assay method of Kunitz (J.Gen.Physiol. 33, 349 and 363 (1950)). A solution in 1 mM Calcium Chloride and 50% (v/v) glycerol.

Formulation

122 u/vial
See application note.

Shipping & Handling

Dry Ice
Store vial at -20° C or colder prior to opening product.
Expiration date is six (6) months from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.