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Deoxyribonuclease I
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Description
Background
Deoxyribonuclease I is an endonuclease which splits phosphodiester linkages yielding polynucleotides with a free 3’-OH and 5’-phosphate. Activated by bivalent metal ions and inhibited by chelators such as EDTA. Chromatographically purified to be free of ribonuclease and protease activity.
Application Note
One unit will cause an increase in the absorbance at 260 nm of 0.001 per minute per ml at 25° C, pH 5.0 when acting on highly polymerized DNA according to the assay method of Kunitz (J.Gen.Physiol. 33, 349 and 363 (1950)). A solution in 1 mM Calcium Chloride and 50% (v/v) glycerol.
Purity/Specificity
Enzyme Concentration: 2290 units/ml or ~2.2 units/ul (100 units/vial) which approximately equals 1 mg/ml. % Ribonuclease: None detected % Protease: None detected
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