CyclinC protein-GST fusion
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Datasheet

CyclinC protein-GST fusion

009-001-R77S 009-001-R77
20 µg 50 µg
Human
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$298.00 /Per Item
$579.00 /Per Item
20 µg $298.00
50 µg $579.00
Availability: Ships in approximately 30 days Availability: Ships in approximately 30 days
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Description

Background

Cyclin C is a member of the cyclin family of proteins and interacts with the cyclin-dependent kinase 8 (CDK8) and induces the phosphorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The mRNA levels of Cyclin C fluctuate during the cell cycle and peak at the G1 phase. Cyclin C acts as a critical regulator of the G0/G1 transition of human Hematopoietic stem cells (1). Cyclin C can also combine with CDK3 to stimulate Rb phosphorylation at S807/811 during the G0/G1 transition, and this phosphorylation is required for cells to exit G0 efficiently (2). Cyclin C Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.

Application Note

CyclinC Protein is stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. CyclinC Protein is suitable for use in Western Blot. Expect a band approximately ~ 55kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Purity/Specificity

Recombinant full-length human CyclinC was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.

CyclinC recombinant protein-GST fusion protein - 009-001-R77
CCNC, CycC, Cyclin-C, SRB11 homolog, hSRB12
CCNC
Human
Sf9 cells
Protein
Recombinant Protein
Liquid (sterile filtered)
0.05 µg/µL
See application note.
Dry Ice
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.