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Datasheet

CRKL protein-HIS Epitope

009-001-R74S 009-001-R74
20 µg 50 µg
Liquid (sterile filtered) Liquid (sterile filtered)
Human
Sf9 cells
$279.00 /Per Item
$470.00 /Per Item
20 µg $279.00
50 µg $470.00
Out of stock
Ships in approximately 30 days
Out of stock
Ships in approximately 30 days
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Product Details

CRKL recombinant protein-HIS Epitope - 009-001-R74
crk-like protein, Crk-like protein
Human
Sf9 cells
Recombinant Protein

Target Details

CRKL
Recombinant full length human CRKL was expressed by baculovirus in Sf9 insect cells using an N-Terminal his epitope. The purity was determined to be >80% by densitometry.
?term=NM_005207 - Gene ID

Application Details

CRKL Protein is stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. CRKL Protein is suitable for use in Western Blot. Expect a band approximately ~ 39kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Formulation

0.2 µg/µL
See application note.

Shipping & Handling

Dry Ice
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Expiration date is one (1) year from date of receipt.
CRKL or v-crk sarcoma virus CT10 oncogene homolog (avian)-like is a protein kinase containing SH2 and SH3 (src homology) domains that activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion (1). It is a substrate of the BCR-ABL tyrosine kinase which plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic (2). CRKL is also required for normal cellular responses to Fgf8, including survival and migration, Erk activation, and target gene expression. CRKL Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Apoptosis/Autophagy, Cancer, Cell Cycle, Receptor Tyrosine Kinases, and WNT Signaling research.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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