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Datasheet

Sars-Cov Nonstructural Protein 13 Antibody

Rabbit Polyclonal

100-401-A54
100 µL
IF
SARS-CoV
Rabbit
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$379.00 /Per Item
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Description

Background

The coronavirus nonstructural protein 13 (nsp13) is one of the SARS-Coronavirus replicase cleaving products encoded by ORF1b. Nsp13 is thought to be part of the viral replication complex, which is associated with intracellular membranes. Nsp13 contains a C-terminal NTPase/Helicase domain and an N-terminal putative zinc-binding motif. Anti-SARS-CoV Nonstructural Protein 13 (nsp13) Antibody is useful for researchers interested in viral research.

Synonyms

rabbit anti-Sars-Cov Nonstructural Protein 13 Antibody, Replicase polyprotein 1a, pp1a antibody, Growth factor-like peptide

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a BSA-coupled synthetic peptide corresponding to the C-terminus (amino acid residues 584-601) of the SARS Coronavirus nonstructural protein 13.

Application Note

This antibody has been tested for use in western blotting, immunofluorescence microscopy and immunoelectron microscopy. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 67 kDa in size corresponding to SARS-CoV nsp13 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37°C.  Infection occurred in PBS/DEAE/2% FCS followed by exchange to EMEM/25mMHEPES/2% FCS. Cells were fixed with PBS/3% PFA.  After washing fixed cells, antibody incubation was performed in PBS/5% FCS for 30 min.

Purity/Specificity

This antibody is directed against SARS-Coronavirus nsp13 protein. The product is neat antiserum. Cross reactivity with homologues from other sources has not been determined.

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Anti-SARS-CoV Nonstructural Protein 13 (nsp13) (RABBIT) Antibody - 100-401-A54
1a
IF
SARS
SARS-CoV
Rabbit
Unconjugated
Polyclonal
Antiserum
Peptide
Liquid (sterile filtered)
Dry Ice
Expiration date is one (1) year from date of opening.
85 mg/mL by Refractometry
None
0.01% (w/v) Sodium Azide
None

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  • Multiple Enzymatic Activities Associated With Severe Acute Respiratory Syndrome Coronavirus Helicase.;2004;Ivanov KA. et al.;PubMed