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Human Buffy Coat Preparation

100 µL
Frozen Cell Pellet
$1,010.00 /Per Item
Availability: Ships in approximately 7 days
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.


Blood is a complex mixture of cells, proteins, metabolites and many other substances.  About 56% of human blood volume is comprised of cells, more than 99% of which are erythrocytes.  Human erythrocytes and thrombocytes (platelets, 0.5% blood components) do not contain nuclei.  Leukocytes (0.3% of blood components), also referred to as peripheral blood mononuclear leukocytes or PBMC, do contain nuclei.  Blood samples may vary widely in leukocyte (granulocytes, lymphocytes and monocytes) concentration depending on the health of the donor. Healthy blood contains less than 107 leukocytes per ml, while blood from an infected donor may have ten fold higher leukocyte concentrations. Buffy coat preparations are useful for researchers interested in immunology and infectious disease research.

Product Details

Human Buffy Coat Preparation - R614-0100
Human Buffy Coat Preparation

Target Details

Buffy coat preparations are composed of leukocytes, trace amounts of red blood cells and other components. Buffy coat preparations are separated after a single low-speed centrifugation to remove the platelet rich plasma, buffy coat layer and erythrocytes (RBC). If desired, you may further purify this preparation of leukocytes by sedimentation using Percoll™ gradient centrifugation.

Application Details

Buffy coat preparations are suitable for use as a source of nucleated peripheral blood mononuclear leukocytes (PBMC). Buffy coat preparations are generally enriched for leukocytes (WBC) and contain trace amounts of red cells and other blood components. This product is prepared in a buffer composed of 20.5 mg/ml Dextrose, 4.2mg/ml Sodium Chloride, 8.0 mg/ml Citric Acid Tri-sodium, adjusted to pH 6.1.

Tissue Data



See application note.

Shipping & Handling

Dry Ice
Store vial at -70° C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles.
Expiration date is one (1) year from date of receipt.

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No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.; This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.