2´-O-Methyl (2´-OMe) Panels

The Role of 2´-OMe Modifications in ASO Drug Design

2′-O-Methyl (2′-OMe) chemical modification of oligonucleotides is a second-generation modification often incorporated into antisense oligonucleotides (ASOs), small interfering RNA (siRNA), aptamers, and messenger RNA (mRNA). OMe modification enhances stability, minimizes off-target binding, and reduces innate immune response, which are key benefits when developing safer, more effective RNA therapeutics. However, excessive incorporation of OMe modification may lower the potency of oligonucleotide drugs, requiring consideration for both pros and cons in drug design.

ModDetect™ 2’-OMe panels feature highly specific antibodies that recognize the OMe chemical modification, detecting nucleic acid drugs independent of sequence. These antibodies support quantitative immunoassays across various modalities (ASOs, siRNA, aptamers, mRNA) and can be used to localize and quantify siRNA strands synthesized with distinct chemical modifications (i.e. when phosphorothioate (PS) is present on both strands, but with MOE on the sense strand and OMe on the antisense strand.)