Conversion to Recombinant Antibody
Conversion to Recombinant Antibody
Hybridoma or Purified Ab Sequencing
Rockland can convert your monoclonal antibody from a traditional hybridoma into a recombinant antibody format. This can be achieved by gene sequencing from an existing hybridoma cell line, or by de novo protein sequencing from purified antibody preparation. Once the antibody sequence is known, antibody genes in various formats can be synthesized and expressed to obtain recombinant antibody protein that is monoclonal and pure. Recombinant antibodies can also be generated from other discovery projects (i.e., immunization campaigns using various animal species), or selected using antibody display libraries generated from naïve (i.e., non-immunized), hyper-immunized animals, or synthetic antibody libraries.
Custom Recombinant Monoclonal Antibodies
Rockland's long heritage and expertise in polyclonal & monoclonal antibody discovery and production now includes the conversion of your antibody into recombinant antibody (rAb) formats. By transitioning to a rAb format, the composition, integrity, production yield, quality and performance reproducibility of your antibody can be improved and assured.
rAbs can be produced in a wide range of formats that include common antibody constant regions (e.g., IgG1 Fc, IgG2 Fc, or others) as well as engineered molecular modifications such as fragments of the antibody (e.g., scFv) and inclusion of molecular tags (e.g., FLAG, polyHis, and a range of other options). Various other options include multi-valency and multi-specificity. We can also functionalize your rAbs via genetic fusions to other proteins or biochemically modify them with enzymes (e.g., horse radish peroxidase, alkaline phosphatase, and others), dyes (e.g., FITC and other fluorescent or chemiluminescent molecules) or other tags and molecules (e.g., biotin, PEG).
Whether your antibody is derived from a hybridoma cell line, a yeast or phage display library, or a de novo protein sequencing effort from a purified antibody preparation, we can improve the production and performance of your antibody by conversion to a rAb format.
(Hybridoma cell line or purified antibody)
Secure, High-Yield, and Customizable
Secure Supply & Immortalize Antibodies
Leverage advanced recombinant technology to provide a stable, continuous supply of high-quality antibodies.Optimize Production
Enhance the yield from low-secreting hybridomas through efficient recombinant expression to scale up production.Innovative Engineering
Customize antibodies to meet specific research and therapeutic objectives, with capabilities to modify class, species, or format.De-Risk Genetic Drift
Recombinant technology stabilizes genetic material, preventing the drift that plagues traditional monoclonal antibody production.Interested in conversion to recombinant antibody?
Talk with us to find out how we can help move your research forward.