Oligonucleotide Conjugation

Rockland offers custom oligonucleotide conjugations to various proteins by common coupling chemistry strategies, producing high-quality, oligo-protein conjugates as a complete solutions provider to the oligo Tx industry. Our expertise in cross-linking chemistries enable our scientists to effectively link many oligonucleotides to proteins, regardless of structure or modifications. The resulting conjugates are chemically stable, and suitable for use in in vitro assays, in vivo assays, and immunization studies. Our quality methods can confirm successful oligo-protein labeling and are included in our custom services. Producing high-quality, oligo-protein conjugates is the cornerstone of Rockland’s anti-oligonucleotide antibody generation expertise and allows us to be a complete solutions provider to the oligonucleotide therapeutic industry.

Available Cross-Linking Chemistries for Oligonucleotides

Thiol-maleimide
Thiol-maleimide
NHS-ester
NHS-ester
Glutaraldehyde
Glutaraldehyde

 

Available Conjugates for Antibodies

Label
Haptens
Label
Peroxidase
Label
Biotin
Label
Alkaline Phosphatase
Label
Glucose Oxidase
Label
β-Galactosidase
Label
Phycoerythrins
Label
DyLight™
Label
ATTO Dyes
Label
Fluorescein
Label
Rhodamine
Label
Texas Red

Antibody Oligo Conjugates in Immuno-PCR

Leverage Rockland's expertise in nucleic acid conjugation for novel detection assays such as immuno-PCR (iPCR). Immuno-PCR is a powerful detection method that combines the specificity of an antibody with the sensitivity of PCR. In immuno-PCR, the target protein is first captured and bound by an antibody that has been conjugated to an oligonucleotide label. The oligonucleotide serves as the target sequence for a subsequent PCR. Because of the efficiency of nucleic acid amplification, iPCR typically results in a 10- to 1,000-fold increase in sensitivity compared to an analogous enzyme-linked immunoassay. This enables the detection of rare analytes in complex samples that are difficult to detect in conventional immunoassays. Due to the similarity to a sandwich ELISA, virtually any antibody suitable for ELISA can be adapted for immuno-PCR. Benefit from first-class antibodies and our oligonucleotide conjugation expertise here at Rockland and contact us for details.