Antibody Conjugation

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Antibody Conjugation

With decades of experience providing protein and antibody conjugation services, as well as producing ready-to-use conjugated proteins and antibodies, we advance a wide range of projects for the research, pharmaceutical, diagnostics, and biotech communities. Whether you need milligrams or grams of conjugated antibody, Rockland is ready to deliver.

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Our Antibody Conjugation Capabilities

Our antibody conjugation services team can create the ideal reagent for your immunofluorescence assay by conjugating your antibody or any of our ready-to-use antibodies to a wide range of classic fluorescent markers and next generation fluorochrome dyes that are designed for multiplex detection.

  • Peroxidase (HRP)
  • Biotin
  • Alkaline Phosphatase (ALP)
  • Glucose Oxidase (GO)
  • β-Galactosidase 
  • Phycoerythrins
  • Fluorescent dyes: ATTO Dye, Fluorescein, DyLight™, Rhodamine, Texas Red
 

Peroxidase Conjugation

We typically perform custom HRP conjugation on up to 20 mg of affinity-, Protein A-, or Protein G purified antibody. The peroxidase is conjugated to antibody through an added -CHO group, and typically yields a ratio of 2:1..

Rhodamine Conjugation

Supply up to 20 mg of antibody and we will conjugate to TRITC through amine groups and purify resulting in a F/P ratio of 1 to 5.

Biotin Conjugation

Conducted on up to 20 mg of antibody, we use a long arm spacer biotin derivative to conjugate biotin to your antibody, with a target purification of 10-15:1.

Interested in antibody conjugation services?

We can help move your program forward—contact us to find out how.

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Related Services

Antibody Purification

Working with polyclonal or monoclonal antibodies, we can use a variety of affinity-based methods and our proprietary workflows to achieve the antibody purity your project requires.

Antibody Fragmentation

Backed by decades of experience generating Fc, Fab, and F(ab’)2 fragments, Rockland’s antibody modification team can optimize and scale up antibody fragmentation to achieve your desired goals.

Qualification & Validation

Let the experienced Quality Control team at Rockland ensure that your proteins and antibodies are ready for use. We work with you to develop and execute an effective QC plan that is informative and adheres to the most rigorous scientific standards.