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WIT-T™ Culture Medium
Description
Background
WIT-T™ Culture Medium supports the expansion of transformed epithelial cells. WIT-T™ Culture Medium has been specifically optimized for the robust culture of human mammary epithelial cells of transformed phenotype (e.g., immortalized cells transformed either with SV40 early region, termed BPLE cells, or with both SV40 early region and H-R as, known as BPLER cells), an important experimental model for the study of tumor cell biology. Additional applications for WIT-T in tumor and normal cell biology can be found in peer-reviewed publications. Manufactured by Cellaria, LLC.
Application Note
Suitable for transformed human breast epithelium and human primary T-ALL blasts. Useful for derivation, maintenance, and expansion of adherent and suspension cultures. This is a defined media, which is serum free, and supports a model of triple negative breast cancer stem cells. Cells were grown in culture using WIT-T and were passaged in accordance with the Media QC SOP. Population doubling values and population doubling rates met internal QC specifications.
Purity/Specificity
Found free of microbial contamination by direct culture: No growth: Pass. Mycoplasma: Negative: Pass. Growth characteristics enabled by media is in accordance with internal QC rates and values: Pass. To make WIT-T Complete Medium, combine the following: 500 mL WIT Basal Medium, 15 mL WIT-T Supplement.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.