Datasheet is currently unavailable. Try again or /CatalogEntryPage/ProductSummary/ContactUsLinkText
Datasheet Protocol

WIT-P™ Culture Medium

515 mL
This product is discontinued


WIT-P™ Culture Medium supports the expansion of human primary mammary epithelial cells for more than 20 passages without the growth arrest that is seen with other primary epithelial cell culture media. In addition, primary mammary epithelial cells grown in WIT-P™ Culture Medium exhibit a more normal luminal pattern of gene expression than cells grown in other epithelial culture media. Additional applications for WIT in tumor and normal cell biology can be found in peer-reviewed publications. Manufactured by Cellaria, LLC.

Product Details

WIT-P™ Culture Medium - CM-0101
media for patient derived cell lines, media for cell lines, cell culture media

Target Details

Found free of microbial contamination by direct culture: No growth: Pass. Mycoplasma: Negative: Pass. Growth characteristics enabled by media is in accordance with internal QC rates and values: Pass. To make WIT-P Complete Medium, combine the following: 500 mL WIT Basal Medium, 15 mL WIT-P Supplement (thawed in a 37°C water bath).

Application Details

Suitable for normal human breast epithelium, normal human prostate epithelium, transgenic mouse prostate cancer, normal mouse prostate epithelium. Useful for derivation, maintenance, and expansion of adherent and spherical cultures. This is a defined media, which is serum free, and maintains luminal cell phenotypes. Cells were grown in culture using WIT-P were passaged in accordance with the Media QC SOP. Population doubling values and population doubling rates met internal QC specifications.


Liquid (sterile filtered)

Shipping & Handling

Wet Ice
Shipped as two components. Upon arrival, store basal medium at 4°C. Store supplement at -20°C. Medium and supplement are stable for a minimum of 3 months from date of receipt when stored as directed.
Expiration date is three (3) months from date of receipt.

Invalid lot number

If you need help finding your CoA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Related products to: WIT-P™ Culture Medium