12 Epitope GST Tag Protein Marker Lysate

SDS Page of 12 Epitope GST Tag Protein lysate

Western Blot of GST tag- 12 epitope protein marker

Datasheet

12 Epitope GST Tag Protein Marker Lysate

Name: 12 Epitope GST Tag Protein Marker Lysate
Brand: Rockland Immunochemicals
SKU: MB-302-0100
Price: 149.0 USD
Availability: InStock

Item No. MB-302-0100

Size: 100 µL

Applications: WB, SDS-PAGE

Expressed in: E. coli

$149.00 /Per Item

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Background

Epitope tags are short peptide sequences easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.

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Specifications for 12 Epitope GST Tag Protein Marker Lysate

Product Details

Description: 12 Epitope GST Tag Protein Marker Lysate - MB-302-0100

Synonyms: Flag-tag marker, GST-tag marker, HA-tag marker, 6XHIS-tag marker, T7-tag marker, cMyc-tag marker, V5-tag marker, S-tag marker, HSV-tag marker, VSV-tag marker, Glu-Glu-tag marker, E-tag marker, Lysate marker, Protein Marker, Loading control, epitope tag marker, positive control marker

Reagent Type: MW Standards

Expressed in: E. coli

Target Details

Purity/Specificity: Loading control lysate made in E. Coli for Western blotting expresses 12 different epitope tags.

Application Details

Tested Applications: SDS-PAGE, WB

Application Note: This 12 Epitope Tag Protein Marker Lysate has been tested in SDS-PAGE and western blot and is designed for use as a positive control for anti-epitope tag antibodies. This E.coli lysate contains a recombinant protein comprised of the following commonly used epitope tags: GST--T7--HSV--cMyc--VSV--Glu-Glu--V5--E-tag--Flag--S tag--HA--6XHis. A 48 kDa recombinant protein is visualized after SDS-polyacrylamide gel electrophoresis and western blotting using an antibody recognizing any of the expressed epitope tags listed above. Thaw the lysate to room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 µL is recommended for western blotting.

Formulation

Physical State: Liquid

Concentration: 5 mg/ ml by modified Lowry assay

Buffer: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing.

Expiration: Expiration date is one (1) year from date of receipt.

Related Protocols

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.