Mouse IgG1 (Gamma 1 chain) ATTO 647N Conjugated Antibody
IF - Anti-MOUSE IgG1 (Gamma 1 chain) ATTO 647N Conjugated
WBM - Anti-MOUSE IgG1 (Gamma 1 chain) ATTO 647N Conjugated
Datasheet is currently unavailable. Try again or CONTACT US
Datasheet

Mouse IgG1 (Gamma 1 chain) ATTO 647N Conjugated Antibody

Goat Polyclonal

610-156-040
500 µg
Dot Blot, WB, IF, IHC
Mouse
Goat
Have a Question? Ask a Specialist
$318.00 /Per Item
Availability: Ships next business day
Shipping info:

$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

Anti-Mouse IgG1 ATTO 647N Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. IgG1 chain constitutes 66% of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Note

Anti-Mouse IgG1 ATTO 647N Antibody has been tested by dot blot and western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Purity/Specificity

Anti-Mouse IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG1 coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum, Mouse IgG and Mouse IgG1. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.

Anti-MOUSE IgG1 (Gamma 1 chain) (GOAT) Antibody ATTO 647N Conjugated (Min Cross Bv, Hu, and Rb Serum Proteins) - 610-156-040
Goat anti-mouse IgG1 antibody ATTO647N conjugation, goat anti-mouse IgG1 (gamma 1) ATTO 647N conjugated antibody
Dot Blot, WB
IF, IHC
Mouse
Goat
IgG1
ATTO 647N
Polyclonal
IgG
Native Protein
Mouse IgG1 heavy chain
1.7 moles AT647N per mole of IgG
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
500 µL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot secondary antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Hruska M et al. Nanoscale rules governing the organization of glutamate receptors in spine synapses are subunit specific. Nat Commun. (2022)
Applications
IF, Confocal Microscopy
Groschner LN et al. A biophysical account of multiplication by a single neuron. Nature (2022)
Applications
IHC, ICC, Histology
Scherer KM et al. SARS-CoV-2 nucleocapsid protein adheres to replication organelles before viral assembly at the Golgi/ERGIC and lysosome-mediated egress. Sci Adv. (2022)
Applications
IF, Confocal Microscopy
Leonte MB et al. Aerial course stabilization is impaired in motion-blind flies. J Exp Biol. (2021)
Applications
IHC, ICC, Histology
Schilling et al. Transcriptional control of morphological properties of direction-selective T4/T5 neurons in Drosophila. Development (2019)
Applications
IF, Confocal Microscopy
Hruska et al. Synaptic nanomodules underlie the organization and plasticity of spine synapses. Nature Neuroscience (2018)
Applications
IF, Confocal Microscopy

Invalid lot number

If you need help finding your CofA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.