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Datasheet

Collagen Type VI Antibody Fluorescein Conjugated

Rabbit Polyclonal

600-402-108
50 µg
Bovine; Human
Rabbit
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Description

Background

Anti-Collagen Type VI Fluorescein Conjugated Antibody is specific for collagen type VI protein. The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively. The protein encoded by this gene is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy.

Synonyms

rabbit anti-Collagen Type VI antibody fluorescein conjugation, FITC conjugated rabbit anti-Collagen Type VI antibody, Collagen alpha-1 (VI) chain, Collagen VI antibody, Human mRNA for collagen VI alpha 1 C terminal globular domain antibody

Immunogen

Collagen Type VI from human and bovine placenta

Application Note

Anti-Collagen Type VI (RABBIT) Fluorescein Conjugated Antibody is suitable for western blot, immunoprecipitation, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.

Purity/Specificity

Anti-Collagen VI has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.  Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.   This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV and V collagens.  Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Storage Condition

Store vial at 4° C prior to restoration.   Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.   Expiration date is one (1) year from date of restoration.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Anti-Collagen Type VI (RABBIT) Antibody Fluorescein Conjugated - 600-402-108
COL6A1
Mammalian
Bovine; Human
Rabbit
Fluorescein (FITC)
Polyclonal
IgG
Native Protein
Lyophilized
Ambient
Expiration date is one (1) year from date of opening.
1.0 mg/mL by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Restore with deionized water (or equivalent)
50µL

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