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CRISPR in a Box™ - Complete Gene Editing Laboratory Kit
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Description
Background
The CRISPR in a Box™ – Complete Gene Editing Laboratory Kit (KGA-0200) utilizes revolutionary CRISPR-Cas technology. The model system provides users with all the components necessary to perform an in vitro gene editing reaction consisting of a CRISPR-Cas12a ribonucleoprotein (RNP) that modifies a segment within the lacZ gene using a single-stranded donor DNA template and a mammalian cell-free extract. The single-stranded donor DNA template contains flanking arms of homology to the target site and directs the integration of a NotI restriction enzyme site. The products of this reaction are transformed into the bacteria Escherichia coli (E. coli) and a visual, phenotypic readout of a blue to white color change is seen. Additional downstream sequencing or restriction enzyme digestion can be used to confirm presence of the integrated NotI site. One of the many advantages of the in vitro system is the ability to analyze all gene editing products from a single reaction without the need of a thermocycler or any other complex instrumentation. Other major advantages of utilizing this system include, but are not limited to, exploring different repair pathways (HDR, NHEJ, etc.), targeting a variety of unique genes, and evaluating the efficiency of diverse Cas proteins.
Application Note
The CRISPR in a Box™ – Complete Gene Editing Laboratory Kit is an easy-to-use kit that contains the latest in gene editing technology. This technology was based on the pioneering discoveries of the Gene Editing Institute, which are currently being used to explore next-generation medical therapies for diseases like cancer and sickle-cell anemia. The CRISPR in a Box™ – Complete Gene Editing Laboratory Kit includes all the components necessary to perform an in vitro gene editing reaction and analyze the outcomes without the use of a thermocycler or any other costly instrumentation. The kits have been developed by teachers and scientists at Delaware Tech, GEI and Rockland Immunochemicals with the goal to provide a comprehensive educational experience with many teachable moments, including experiment assembly, cleavage, gene editing and reaction analysis.
Purity/Specificity
This kit contains components sufficient for 10 reactions. See kit protocol for complete information.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.